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[#BE0325]InVivoMAb anti-West Nile/dengue virus E protein

관리자 2020-03-12 Views 1,614

About InVivoMAb anti-West Nile/dengue virus E protein

The E60 monoclonal antibody reacts with West Nile virus (WNV) and dengue virus (DENV) envelope (E) protein. WNV and DENV are small, enveloped, single-stranded RNA viruses of the family Flaviviridae. The viral envelope of flaviviruses consists of multiple copies of the 50-60 kDa E protein and the 8 kDa M (membrane) protein. X-ray crystallography studies have revealed that the ectodomain of each E protein is comprised of three structural domains: DI, DII and DIII, connected by flexible hinges. The tip of the DII domain contains a conserved region termed the “fusion loop,” which is required for membrane fusion of the viral envelope with the host cell membrane. The E60 antibody is a flavivirus cross-reactive neutralizing antibody that binds to an epitope in the fusion loop peptide of domain II on the E protein.


InVivoMAb anti-West Nile/dengue virus E protein Specifications

Isotype

Mouse IgG2a, κ

Recommended Isotype Control(s)InVivoMAb mouse IgG2a isotype control, unknown specificity(BE0085)
Recommended InVivoPure Dilution BufferInVivoPure pH 7.0 Dilution Buffer(IP0070)
Immunogen

Purified soluble WNV E protein

Reported Applications
  • Neutralization of WNV
  • Neutralization of DENV1,2,3,4
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein A

Molecular Weight

150 kDa

RRID

AB_2819052

Storage

The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.


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