The 1A8-CP129 monoclonal antibody is a chimeric version of the original 1A8 antibody. The variable domain sequences are identical to the original 1A8 but the constant region sequences have been switched from rat IgG2a to mouse IgG2a. The 1A8-CP129 antibody contains no Fc mutations just as the original rat IgG2a antibody does not. The 1A8-CP129 monoclonal antibody reacts with mouse Ly6G. Ly6G is a 21-25 kDa member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Ly6G is expressed differentially during development by cells in the myeloid lineage including monocytes, macrophages, granulocytes, and neutrophils. Monocytes typically express Ly6G transiently during development while mature granulocytes and peripheral neutrophils retain expression making Ly6G a good cell surface marker for these populations. Unlike the RB6-8C5 antibody, the 1A8-CP129 antibody reacts specifically with mouse Ly6G with no reported cross reactivity with Ly6C.

See the references for the original rat IgG2a 1A8 antibody (https://bioxcell.com/catalogsearch/result/?q=BP0075-1).