RecombiMAb anti-mouse CD4

Clone GK1.5-CP127
Catalog # CP127
Category in vivo Recombinant Antibodies
Price
Size Regular Price
1mg $ 217.00
5mg $ 843.00
25mg $ 2,942.00
50mg $ 4,229.00
100mg $ 5,910.00
About RecombiMAb anti-mouse CD4

The GK1.5-CP127 monoclonal antibody is a chimeric version of the original GK1.5 antibody. The variable domain sequences are identical to the original GK1.5 but the constant region sequences have been switched from rat IgG2b to mouse IgG2b. The GK1.5-CP127 antibody contains no Fc mutations just as the original rat IgG2b antibody does not. The GK1.5-CP127 monoclonal antibody reacts with the mouse CD4. The CD4 antigen is a 55 kDa cell surface type I membrane glycoprotein belonging to the immunoglobulin superfamily. CD4 acts as a co-receptor which in cooperation with the T cell receptor (TCR) interacts with class II MHC molecules displayed by antigen presenting cells (APC). CD4 is expressed by the majority of thymocytes, most helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally.

See the references for the original rat IgG2b GK1.5 antibody (https://bioxcell.com/catalogsearch/result/?q=BP0003-1). 

RecombiMAb anti-mouse CD4 Specifications
IsotypeMouse IgG2b, κ
Recommended Isotype Control(s)InVivoPlus mouse IgG2b isotype control, unknown specificity
Recommended Dilution BufferInVivoPure pH 6.5 Dilution Buffer
ImmunogenMouse CTL clone V4
Reported Applicationsin vivo CD4+ T cell depletion*
Flow cytometry*
Western blot*
*Reported for the original rat IgG2b GK1.5 antibody
FormulationPBS, pH 6.5
Contains no stabilizers or preservatives
Endotoxin<1EU/mg (<0.001EU/μg)
Determined by LAL gel clotting assay
Aggregation<5%
Determined by DLS
Purity>95%
Determined by SDS-PAGE
Sterility0.2 µm filtration
ProductionPurified from CHO cell supernatant in an animal free facility
PurificationProtein G
Molecular Weight150 kDa
Murine Pathogen TestsEctromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
Application References