The 2.11 monoclonal antibody reacts with an epitope in the Cr4 domain of TCR Vγ1.1 (T cell receptor V gamma 1.1). The TCR is expressed on the surface of T lymphocytes and is responsible for recognizing fragments of antigen as peptides bound to MHC molecules. When the TCR engages with antigenic peptide and MHC the T lymphocyte is activated through signal transduction. The Vγ1Jγ4Cγ4 chain is expressed by a major population of γδ T cells in the thymus and peripheral lymphoid organs of adult mice. However, during postnatal and early life stages only a minor population of γδ T cells express Vγ1Jγ4Cγ4 during fetal and early postnatal life.

INVIVOMAB ANTI-MOUSE TCR VΓ1.1/CR4 (CLONE: 2.11)

Narayan, K., et al. (2012). “Intrathymic programming of effector fates in three molecularly distinct gammadelta T cell subtypes.” Nat Immunol 13(5): 511-518. PubMed

Innate gammadelta T cells function in the early phase of immune responses. Although innate gammadelta T cells have often been studied as one homogenous population, they can be functionally classified into effector subsets on the basis of the production of signature cytokines, analogous to adaptive helper T cell subsets. However, unlike the function of adaptive T cells, gammadelta effector T cell function correlates with genomically encoded T cell antigen receptor (TCR) chains, which suggests that clonal TCR selection is not the main determinant of the differentiation of gammadelta effector cells. A high-resolution transcriptome analysis of all emergent gammadelta thymocyte subsets segregated on the basis of use of the TCR gamma-chain or delta-chain indicated the existence of three separate subtypes of gammadelta effector cells in the thymus. The immature gammadelta subsets were distinguished by unique transcription-factor modules that program effector function.