RecombiMAb anti-mouse PD-L1 (B7-H1)

Clone 10F.9G2-CP168
Catalog # CP168
Category in vivo Recombinant Antibodies
Price
Size Regular Price
1mg $ 217.00
5mg $ 843.00
25mg $ 2,942.00
50mg $ 4,229.00
100mg $ 5,910.00
About RecombiMAb anti-mouse PD-L1 (B7-H1)

The 10F.9G2-CP168 monoclonal antibody is a chimeric version of the original 10F.9G2 antibody. The variable domain sequences are identical to the original 10F.9G2 but the constant region sequences have been switched from rat IgG2b to mouse IgG1. The 10F.9G2-CP168 antibody contains no Fc mutations just as the original rat IgG2b antibody does not. The 10F.9G2-CP168 antibody reacts with mouse PD-L1 (programmed death ligand 1) also known as B7-H1 or CD274. PD-L1 is a 40 kDa type I transmembrane protein that belongs to the B7 family of the Ig superfamily. PD-L1 is expressed on T lymphocytes, B lymphocytes, NK cells, dendritic cells, as well as IFNγ stimulated monocytes, epithelial cells and endothelial cells. PD-L1 binds to its receptor, PD-1, found on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Engagement of PD-L1 with PD-1 leads to inhibition of TCR-mediated T cell proliferation and cytokine production. PD-L1 is thought to play an important role in tumor immune evasion. Induced PD-L1 expression is common in many tumors and results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and PD-1. The 10F.9G2 antibody has been shown to block the interaction between PD-L1 and PD-1 and between PD-L1 and B7-1 (CD80). 

RecombiMAb anti-mouse PD-L1 (B7-H1) Specifications
IsotypeMouse IgG1, κ
ImmunogenMouse CD274
Reported Applications
  • in vivo PD-L1 blockade*
  • Immunofluorescence*
  • Immunohistochemistry (frozen)*
  • Flow cytometry*
  • Western blot*

*Reported for the original rat IgG2a RMP1-14 antibody

Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Endotoxin
  • <1EU/mg (<0.001EU/μg)
  • Determined by LAL gel clotting assay
Aggregation
  • <5%
  • Determined by DLS
Purity
  • >95%
  • Determined by SDS-PAGE
Sterility0.2 μM filtered
ProductionPurified from CHO cell supernatant in an animal free facility
PurificationProtein A
Molecular Weight150 kDa
Murine Pathogen Test Results
  • Mouse Norovirus: Negative
  • Mouse Parvovirus: Negative
  • Mouse Minute Virus: Negative
  • Mouse Hepatitis Virus: Negative
  • Reovirus Screen: Negative
  • Lymphocytic Choriomeningitis virus: Negative
  • Lactate Dehydrogenase-Elevating Virus: Negative
  • Mouse Rotavirus: Negative
  • Theiler’s Murine Encephalomyelitis: Negative
  • Ectromelia/Mousepox Virus: Negative
  • Hantavirus: Negative
  • Polyoma Virus: Negative
  • Mouse Adenovirus: Negative
  • Sendai Virus: Negative
  • Mycoplasma Pulmonis: Negative
  • Pneumonia Virus of Mice: Negative
  • Mouse Cytomegalovirus: Negative
  • K Virus: Negative
StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
Application References

RECOMBIMAB ANTI-MOUSE PD-L1 (B7-H1)