RecombiMAb anti-mouse PD-1 (CD279) (LALA-PG)

Clone RMP1-14-CP153
Catalog # CP153
Category in vivo Recombinant Antibodies
Price
Size Regular Price
1mg $ 217.00
5mg $ 843.00
25mg $ 2,942.00
50mg $ 4,229.00
100mg $ 5,910.00
About RecombiMAb anti-mouse PD-1 (CD279) (LALA-PG)

The RMP1-14-CP153 monoclonal antibody is a chimeric version of the original RMP1-14 antibody. The variable domain sequences are identical to the original RMP1-14 but the constant region sequences have been switched from rat IgG2a to mouse IgG2a. The RMP1-14-CP153 antibody also contains a LALA-PG mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. RMP1-14-CP153 reacts with mouse PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1’s structure includes a ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 and PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T-cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance and prevention of autoimmune disease in mice as PD-1 knockout animals show dilated cardiomyopathy, splenomegaly, and loss of peripheral tolerance. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being explored as cancer treatments.

See the references for the original rat IgG2a RMP1-14 antibody (https://bioxcell.com/invivoplus-anti-mouse-pd-1-cd279-bp0146). 

RecombiMAb anti-mouse PD-1 (CD279) (LALA-PG) Specifications
IsotypeMouse IgG2a, κ
Recommended Isotype Control(s)RecombiMAb mouse IgG2a (D265A) isotype control, anti-hen egg lysozyme
Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer
MutationsL234A, L235A, P329G (LALA-PG)
ImmunogenSyrian Hamster BKH cells transfected with mouse PD-1 cDNA
Reported Applicationsin vivo blocking of PD-1/PD-L signaling*
*Reported for the original rat IgG2a RMP1-14 antibody
FormulationPBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin<1EU/mg (<0.001EU/μg)
Determined by LAL gel clotting assay
Aggregation<5%
Determined by DLS
Purity>95%
Determined by SDS-PAGE
Sterility0.2 µm filtration
ProductionPurified from CHO cell supernatant in an animal free facility
PurificationProtein G
Molecular Weight150 kDa
Murine Pathogen TestsEctromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
Application References